326. A Versatile Tool for Conditional Gene Expression and Knockdown
نویسندگان
چکیده
منابع مشابه
A versatile genetic tool for post-translational control of gene expression in Drosophila melanogaster
Several techniques have been developed to manipulate gene expression temporally in intact neural circuits. However, the applicability of current tools developed for in vivo studies in Drosophila is limited by their incompatibility with existing GAL4 lines and side effects on physiology and behavior. To circumvent these limitations, we adopted a strategy to reversibly regulate protein degradatio...
متن کاملA Versatile Computational Tool for Observed Variable Mediation , Moderation , and Conditional Process Modeling
Statistical mediation and moderation analysis are widespread throughout the behavioral sciences. Increasingly, these methods are being integrated in the form of the analysis of ―mediated moderation‖ or ―moderated mediation,‖ or what Hayes and Preacher (in press) call conditional process modeling. In this paper, I offer a primer on some of the important concepts and methods in mediation analysis...
متن کاملA Cre-loxP-based mouse model for conditional somatic gene expression and knockdown in vivo by using avian retroviral vectors.
Site- and time-specific somatic gene transfer by using the avian sarcoma-leukosis retrovirus RCAS (replication-competent avian sarcoma-leukosis virus long terminal repeat with splice acceptor) has been shown to be a powerful tool to analyze gene function in vivo. RCAS retroviruses that express the avian subgroup A envelope transduce only mammalian cells genetically engineered to express the avi...
متن کاملFlpStop, a tool for conditional gene control in Drosophila
Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cell division. Here we describe FlpStop, a generalizable tool for conditional gen...
متن کاملA versatile vector system for multiple gene expression in plants.
Today, cloning vectors that have been specifically designed to facilitate the fusion, overexpression or down-regulation of a variety of genes in plant cells are available from various sources. In most cases, their basic design allows the cloning of a single target gene, typically under a specific promoter, in parallel with the expression of selection and/or marker genes from the same vector. Ho...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2006
ISSN: 1525-0016
DOI: 10.1016/j.ymthe.2006.08.383